What is GIANI?
Giani is a FIJI plugin for the routine analysis of 3D cell biology image data. Implemented specifically with batch-processing in mind, GIANI is designed to:
Read data sets in any format recognised by BioFormats
Identify centres of nuclei using blob detection
Segment nuclei fully, using either mcib3d or MorphoLibJ
Segment cells
Measure nuclear and cell morphology, together with fluorescence intensities within these regions.
Explore results using a results browser based on the 3D RoiManager
Click here to read the paper introducing GIANI.