What is GIANI?

Giani is a FIJI plugin for the routine analysis of 3D cell biology image data. Implemented specifically with batch-processing in mind, GIANI is designed to:

  • Read data sets in any format recognised by BioFormats

  • Identify centres of nuclei using blob detection

  • Segment nuclei fully, using either mcib3d or MorphoLibJ

  • Segment cells

  • Measure nuclear and cell morphology, together with fluorescence intensities within these regions.

  • Explore results using a results browser based on the 3D RoiManager

Click here to read the paper introducing GIANI.